Combine Graphene Biosensors together with Nanoparticles and/or Bacteria in an SPR device?

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Enter the world of Graphene Biosensing in Surface Plasmon Resonance with SENSIA:
SENSIA is a technological leader company  in the field of analytical instrumentation based on SPR (Surface Plasmon Resonance), for life sciences laboratories and environmental measurements: 
SENSIA is breaking through into the SPR market with the first commercially available graphene biosensors , enabling new extreme unattained sensitivities in SPR.
Additionally, the design of microfluidics allows the perfect use of nanoparticles and /or bacteria, for new applications and uses of the SPR technology.
Graphene Biosensing:
Gold Biosensors and Graphene coated Biosensors are commercially available and can be indifferently used in the Indicator-G (TM), a new device by Sensia, opening new paths for Research .
The technologies developed by SENSIA are based on SPR (Surface Plasmon Resonance) technology.

 SENSIA has been following  an innovative process of machine development and is presently bringing into market a new fully automated,  bi-channel  SPR device, delivering with an unequalled sensitivity, transportability, robustness, and extreme stability, allowing deepen existing conventional SPR techniques into the High–SPR world. Its name: the Indicator (TM).


Oleksandr Zagorodko , Julie Bouckaert , Tetiana Dumych , Rostyslav Bilyy , Iban Larroulet , Aritz Yanguas Serrano , Dimitri Alvarez Dorta , Sebastien G. Gouin , Stefan-Ovidiu Dima , Florin Oancea , Rabah Boukherroub , and Sabine Szunerits

reference: Biosensors 2015,
5(2), 276-287;doi:10.3390/bios5020276 (registering DOI)
Received: 30 April 2015 / Accepted: 19 May 2015 / Published: 26 May 2015

Abstract: The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner. We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min−1), and reaches a maximum at flow rates of 30 µL·min−1 (≈30 mPa). This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.
(This article belongs to the Special Issue "Affinity Sensors")



Highly sensitive detection of DNA hybridization on commercialized graphene coated surface plasmon resonance interfaces

reference:     Anal. Chem.,  2014,  86,  11211-11216. PDF

Institut de Recherche Interdisciplinaire, USR 3078 CNRS, Université Lille 1, Parc de la Haute Borne, 50 Avenue de Halley, BP 70478, 59658 Villeneuve d’Ascq, France
Laboratoire de Réactivité de Surfaces, UMR CNRS 7197, Université Pierre et Marie Curie—Paris VI, Site d’Ivry−Le Raphaël, 94200 Ivry-sur-Seine, France
§ SENSIA SL, Poligono Aranguren, 9, Apartado de Correos 171, 20180 Oiartzun, Gipuzkoa, Spain
Graphenea S.A., Tolosa Hiribidea, 76, 20018 Donostia, San Sebastian, Spain

Anal. Chem.,  2014,  86,  11211-11216. PDF

Copyright © 2014 American Chemical Society


Strategies employed to interface biomolecules with nanomaterials have advanced considerably in recent years and found practical applications in many different research fields. The construction of nucleic acid modified interfaces together with the label-free detection of hybridization events has been one of the major research focuses in science and technology. In this paper, we demonstrate the high interest of graphene-on-metal surface plasmon resonance (SPR) interfaces for the detection of DNA hybridization events in the attomolar concentration range. The strategy consists on the non-covalent functionalization of graphene coated SPR interfaces with gold nanostars carrying single-stranded DNA (ssDNA). Upon hybridization with its complementary, desorption of the nanostructures takes place and thus enables the sensitive detection of the DNA hybridization event. The DNA sensor exhibits a detection limit of ≈500 aM for complementary DNA with a linear dynamic range up to 10-8 M. This label-free DNA detection platform should spur off new interest towards the use of commercially available graphene-coated SPR interfaces.





Plasmonic Photothermal Therapy of uropathogenic E. coli with reduced graphene oxide and core/shell nanocomposites of gold nanorods/reduced graphene oxide

 reference:  J. Mater. Chem. B, 2015, 3, 375     

Received 23rd October 2014, Accepted 19th November 2014 ,

DOI: 10.1039/c4tb01760a, B

Kostiantyn Turcheniuk, Charles-Henri Hage, Jolanda Spadavecchia, Aritz Yanguas Serrano, Iban Larroulet, Amaia Pesquera, Amaia Zurutuza, Mariano Gonzales Pisfil, Laurent Heliot, Julie Bouckaert, Rabah Boukherroub and Sabine Szunerits 


The development of non antibiotic based treatments against bacterial infections by gram-negative uropathogenic E. coli is a complex task. New strategies to treat such infections are thus urgently needed. This report illustrates the development of pegylated reduced graphene oxide nanoparticles (rGO-PEG) and gold nanorods (Au NRs) coated with rGO-PEG (rGO-PEG-Au NRs) for the selective killing of uropathogenic E. coli UTI89. We took advantage of the excellent light absorption properties of rGO-PEG and Au NRs particles in the near-infrared (NIR) to photothermally kill gram-negative pathogens up to 99% in 10 min. by illumination of solutions containing the bacteria. The rGO-PEG-Au NRs demonstrated better photothermal efficiency towards E. coli than rGO-PEG. Targeted killing of E. coli UTI89 could be achieved with rGO-PEG-Au NRs functionalized with multimeric heptyl α-D-mannoside probes. This currently offers a unique biocompatible method for the ablation of pathogens with the opening of probably a new possibility for clinical treatments of patients with urinary infections.


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