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References

Applications


Several biomolecular interactions have been explored in SPR sensors as, for example, antibody-antigen, receptor-ligand or hormone-receptor because a wide variety of affinity ligands can be used: antibodies, antibody fragments, genetically engineered antibody derivatives, lectins, membrane receptors, nucleic acids, gene fusion protein, hormones, viruses, bacteria,…

System Built-Up

 
A SPR sensor based in the Kretschmann configuration is used in this instrument to achieve the resonant condition by total internal reflection. A polarised laser diode light strikes into two flow cells, with a volume of 300nl.  A prism and a multi-photodiode are located on concentric rotary stages with an angular resolution of 0.01º. β-SPR system has a detection limit of 10-5 in refractive index.

Replaceable sensor glass slides are 10 mm by 10mm coated with a 50nm Au layer. Self assembled monolayers or thin films add the surface chemistry functionality required for the sensor surface to detect a binding partner.

Monitoring changes in refractive index as a function of time allows real-time analysis of the binding events occurring at the sensor surface due to the continuous flow of buffers and samples provided by the flow injection system.